Victor Barrio, a 29-year-old professional bullfighter, was killed when a bull's horn pierced his chest in front of spectators as he competed in a fight in the town of Teruel in the eastern region of Aragon.
His death, shown live on television, was confirmed on the website of Madrid's Las Ventas bullring, where Barrio began as an apprentice bullfighter in 2010. He is the first Spanish bullfighter to die in a ring since the turn of the century.
The last matador killed in bullfighting was Jose Cubero Yiyo in 1985, whereas the last death taking place in Spanish bullrings was in 1992 when Ramon Soto Vargas, the "banderillero" who places colorful sticks on the top of the bull's shoulder, was killed.
In the southeastern village of Pedreguer near Valencia, a 28-year-old Spaniard was killed during a bull-run, in which people risk life and limb by racing alongside specially-bred fighting bulls through narrow streets.
A bull's horn pierced his lung and heart as he was trying to help another runner during the event, in which a man was killed last year, a spokesman for the regional government said. He was taken by ambulance to a nearby hospital and pronounced dead an hour later.
The town suspended all bull-related festivities for the day.
Many of Spain's towns hold summer festivals involving bulls, and several people die each year.
The San Fermin festival, in which bulls chase red-scarved runners through Pamplona's cobbled streets during nine days of events, attracts thousands of revelers from Spain and overseas.
In Saturday's run there, a 33-year-old Japanese man was gored in the chest and a 24-year-old Spanish man in the arm, while 12 others suffered minor injuries, the local government said on its website.
The Japanese man was in a stable condition in hospital, a spokesman for the festival said.
The bull run was unusually long, with one bull left stranded at the starting gate, where he proceeded to charge and strike a couple of runners. Many other participants fell and were stampeded by the head of the pack in the 930-yard (850-meter) race.
Another of the six bulls in the run got separated from the pack, did a U-turn and gored a nearby runner, lifting his body off the ground and flipping him over.
The four-minute run in Pamplona featured six bulls from the Jose Escolar ranch, one of which separated from the rest and caused panic among the runners.
The daily bull-run along an 825-metre stretch of narrow streets in Pamplonatarget="_blank"'>